Potential Role of Lipometabolism-Related MicroRNAs in Peripheral Blood Mononuclear Cells as Biomarkers for Coronary Artery Disease
نویسندگان
چکیده
AIM To explore the relationship between lipometabolism-related microRNAs (miRNAs) in peripheral blood mononuclear cells (PBMCs) and the presence of coronary artery disease (CAD). METHODS In the present study, 161 stable CAD patients and 149 health controls were enrolled. The expression levels of seven miRNAs (miR-21, miR-24, miR-29a, miR-33a, miR-34a, miR-103a, and miR-122) in PBMCs were qualified by quantitative real-time polymerase chain reaction (qRT-PCR). The miRNA markers that showed significant difference between the two groups were used for further analysis. The risk of miRNA contributing to the presence of CAD was estimated by univariate and multivariate logistic regression models. The area under the receiver operating characteristic curve (AUC) was used to evaluate diagnostic accuracy. RESULTS The expression levels of miR-24, miR-33a, miR-103a, and miR-122 in PBMCs were significantly increased in CAD patients compared with controls and were significantly correlated with blood lipids in both CAD patients and controls. The increased levels of miR-24 (adjusted OR=1.32, 95% CI 1.07-1.62, P=0.009), miR-33a (adjusted OR=1.57, 95% CI 1.35-1.81, P<0.001), miR-103a (adjusted OR=1.01, 95% CI 1.01-1.02, P<0.001), and miR-122 (adjusted OR=1.03, 95% CI 1.01-1.04, P<0.001) were associated with risk of CAD. We identified a miRNA panel (miR-24, miR-33, miR-103a, and miR-122) that provided a high diagnostic accuracy of CAD (AUC=0.911, 95% CI 0.880-0.942). CONCLUSION The increased expression levels of miR-24, miR-33a, miR-103a, and miR-122 in PBMCs are associated with risk of CAD. A panel of the four miRNAs has considerable clinical value in diagnosing stable CAD.
منابع مشابه
Resting and Activated Natural Tregs Decrease in the Peripheral Blood of Patients with Atherosclerosis
Background: Atherosclerosis is a chronic inflammatory disease affecting large and medium arteries. CD4+ T cells are known to play a role in the progression of the disease. CD4+CD25+Foxp3+ natural Treg (nTreg) cells seem to have a protective role in the disease and their reduction in acute coronary syndrome is recently shown. Objective: To investigate the frequency of nTreg subsets in the pe...
متن کاملEffects of Taurine, Sestrin 2 and Phyllanthin on coronary artery diseases
Heart failure is a growing epidemic in the worldwide. Atherosclerosis is a major mechanism of cardiovascular disease including myocardial infarction and peripheral arterial disease. Moreover, it causes many diseases and deaths around the world. Atherosclerosis, like coronary artery disease (CAD), is associated with inflammation and oxidative stress. The current article has been collected the s...
متن کاملThe Effect of Aquatic and Dryland Resistance Training on Peroxisome Proliferator Activated Receptor-ɑ Gene Expression in Middle-aged Women’s Peripheral Blood Mononuclear Cell after Coronary Artery Bypass Grafting
Background. Cardiac rehabilitation program is aimed at reducing secondary risk factors and improving function in patients undergoing coronary artery bypass grafting run which ultimately may delay or reduce mortality in patients. A major component of cardiac rehabilitation program is exercise. Objective(s). This study is aimed at evaluating the effect of aquatic and dryland training on Perox...
متن کاملEvaluation of Extracellular Circulating Human MicroRNA-197 as a Target Biomarker in Patients with Coronary Artery Disease
Background: Coronary Artery Disease (CAD) refers to the reduction or blockage of all or part of the coronary arteries due to the process of atherosclerosis or the presence of a clot. The aim of this study was to investigate the association of serum miR-197 as a diagnostic index in patients with coronary artery disease. Methods: In this study, 100 patients with CAD were selected. Extraction of...
متن کاملCharacterization of levels and cellular transfer of circulating lipoprotein-bound microRNAs.
OBJECTIVE MicroRNAs are important intracellular regulators of gene expression, but also circulate in the blood being protected by extracellular vesicles, proteins, or high-density lipoprotein (HDL). Here, we evaluate the regulation and potential function of HDL- and low-density lipoprotein-bound miRs isolated from healthy subjects and patients with coronary artery disease. APPROACH AND RESULT...
متن کامل